JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD
II Year B. Tech. Biotech I-Sem L T/P/D C
4 -/-/- 4
BIOCHEMISTRY

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Objectives: The major objective of this course is to provide exposure to students with the chemical nature of biomolecules both simple and complex and to impart an understanding of important metabolic pathways and their regulations.

UNIT - I

Fundamentals of Biochemistry:
Chemical foundations of Biology: Properties of water, pH & Buffers, The Handerson Hasselbalch equation, determination of pKa values, Physiological buffers. Covalent bond and non covalent bond interactions. Classes of organic compounds and functional groups – Basics of Stereochemistry of Biomolecules.
Bioenergetics; Redox potential, components in electron transport systems in mitochondria, respiratory chain. Oxidative phosphorylation - Energetics ,High energy compounds.

UNIT - II

Carbohydrates:
Classification of carbohydrates,properties and chemical reactions of carbohydrates,Industrially significant carbohydrates, Lectins,Glucose tolerance test,Glycolysis,Aerobic and anaerobic fate of Pyruvate, Gluconeogenesis,Pentose phosphate pathway

UNIT - III

Proteins:
Amino acids and peptides - classification, chemical reactions and physical properties, non- natural aminoacids and their significance.
Proteins - classification and hierarchy in structure, Ramachandran map,Nitrogen cycle, Nitrogen balance, Reductive amination and transamination, Urea cycle; Production of aminoacids-Glutamate pathway, Shikimate pathway for the production of aminoacids.

Unit - IV

Lipids:
Classification of lipids, Characterisation of Fats, Brief outlines of Fatty acid synthesis and breakdown, Significance of cholesterol and lipoproteins in Lipid profile analysis,Lipids as surfactants in industry.

UNIT - V

Nucleic Acids: 
Nucleic acids and their metabolism, Structure of purines and Pyrimidines,Brief outlines of Biosynthesis and Degradation.

TEXT BOOKS:

1. Lehninger A L,Nelson O.L,M.M.Cox,Principles of Biochemistry 3rd edition,2000CBS Publicatons.
2. L.Stryer ,JM.Berg ,JL Tymockzo Biochemistry 5th edition,WH Freeman & Co ,2002

REFERENCES:

1. Voet D,Voet J .G.Biochemistry,second Edition,John C wiley and Sons,1994
2. Campbell and Farell ,Biochemistry ,Brooks/cole CENGAGE learning ,2008
3. Fundamentals of Biochemistry JL.Jain S.chand Publishers.

Outcomes: After the completion of the course, the student will gain an understanding of the metabolic processes, their control and how the stoichiometry of metabolites influences the production of biotech products.