JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD
II Year B.Tech. BME. I-Sem L T/P/D C
4 -/-/- 4
APPLIED BIOCHEMISTRY

UNIT - I

Properties of water: pH & Buffers, Physiological buffer. The Handerson Hasselbalch equation, determination of pKa values. Structural aspects of carbohydrates , amino acids and lipids. Carbohydrate metabolism: respiration-types. glycolysis and kreb’s cycle and energetics involved, Protein & Lipid metabolism (Briefly).

UNIT - II

Biochemistry of Living Cell: types of cells, pro and eu karyotes. Sub-cellular - Fractionation using the Differential Centrifugation Method. Functions of each Organelle, Chemical Composition of cell walls Membrane lipids. Transport of Substances across Biological Membrane. Redox potential, components in electron transport systems in mitochondria, respiratory chain. Oxidative phosphorylation - Energetics ,

UNIT - III

Chemical nature of Enzymes - study of the Properties of Enzymes and kinetics by Spectrophotometer. Diagnostic and therapeutic uses of Enzymes. Metalions in biological catalysis (explanation with few examples).

UNIT - IV

Nucleic Acid chemistry: Protein synthesis. Transcription and Translation, Replication, Polymerase Chain Reaction(PCR) Immunological Techniques or Immunoassay – Radio Immuno Assay (RIA), Enzyme- Linked Immunosorbent Assay (ELISA), Chemiluminiscence.

UNIT - V

Blood Chemistry: Chemical Composition of Blood, Separation of Serum Proteins and lipoproteins by Electrophoresis and Ultracentrifugation Acid Base Balance and Biochemical Measurements of Acid-Base and Electrolyte status of the patients, Urine Analysis. General methods of biochemical analysis carried out in the estimation of blood constituents, such as glucose etc. Principles and different methods of chromatography – fluorometry, flame photometry, Automation and Biochemical Analysis. Applications of isotopes in biochemistry.

TEXT BOOKS:

1. Lehninger A.L, Nelson O.’L. M.M. Cox, Principles of Biochemistry 3rd edition, 2000 CBS
2. Robert Murray, Peter A. Mayes, Victor W. Rodwell, Daryl K. Granner, Harper’s Biochemistry, 26th Edition, McGraw-Hill Companies, February 2003.

REFERENCES:

1. Rao, N. Mallikarjuna, Medical Biochemistry, New Age International Pvt Ltd,2002.
2. Lalit M. Srivastava, Nibhriti Das & Subrata sinha, Essentials of practical Biochemistry, CBS Publishers, Ist edition, 2002.
3. Bertini I, Gray HB, Stiefel EI, Valentine JS Biological inorganic chemistry. University Science Books, Sausalito, 2006.
4. Frausto da Silva JJR, Williams RJP The biological chemistry of the elements: the inorganic chemistry of life. Oxford University Press, New York, 2001
5. Bertini I, Sigel A, Sigel H Handbook on metalloproteins. Marcel Dekker, New York ,2001.