JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD
II Year B.Tech. Chem Engg -I Sem L T/P/D C
4 -/-/- 4
ANALYTICAL CHEMISTRY

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Objective: To import the knowledge on principles of analytical methods, spectroscopy & chromatography techniques.

UNIT - I

Principles of Analytical Methods: Quantitative analysis - Gravimetry : Precipitation, types of precipitates, impurities, co-precipitation , post-precipitation, conditions for participation, precipitation from homogeneous solution Gravimetric determination of Fe, Ni and Cu, calculations, Volumetry : Acid base titrations: Indicators; Oxidation-reduction titrations; Complexation using ligands, complexometric titration with EDTA, metal ion indicators; simple calculations; analysis of Na₂CO₃, Fe₂O₃, Brass, Solder etc.

UNIT - II

Molecular Spectrophotometry: UV-Visible Spectroscopy: Absorption spectra, Lamberts Law, Beer’s Law - Combined law equation; Derivations from Beer’s Law. Block diagram of a uv- visible spectrophotometer – quantitative analysis; Direct method for the determination of metal ions; Chromium, Manganese, Iron etc in alloys; Infrared Spectroscopy : Interaction of infra-red radiation with molecules, Sources of IR Radiation; Spectral regions; Block diagram of IR Spectrometer , Function of each component; Sampling Techniques; Application of IR Spectroscopy to functional group analysis ( -OH, -NH₂, -CHO, -CO-R, -CONH).

UNIT - III

Chromatography: Principles, planar chromatography, paper chromatography, RF value. Thinlayer chromatography, identification of spots by spraying and other methods; Gas Chromatography: Principles of Gas Chromatography, block diagram of gas chromatograph, Function of each component, Detectors (FID, ECD), stationary phase for column, mobile phase, chromatogram, qualitative analysis, quantitative analysis, retention time, retention volume, capacity factor, area., normalization method; HPLC: Principles of high performance liquid chromatography, Block diagram of HPCL, Systems, functions of each component, stationary phases, eluting solvents, pumps, detectors, quantitative applications of HPLC.

UNIT - IV

Thermal Analysis: Introduction – Types of thermo gravimetric analysis – principle and method – Instrumentation – Derivative thermo gravimetric analysis – Applications. Differential thermal analysis – Principle of working – Simultaneous DTA – TGA curves – Factors effecting results –Applications.

UNIT - V

Analysis of water: Hardness, definition, Types of Hardness, estimation of hardness by EDTA method, Alkalinity, Acidity, Chlorides, Chlorine, dissolved oxygen, BOD, COD.

TEXT BOOK

1. Quantitative analysis, R.A. Day & A.L.Underwood Printice-Hall of India, pvt. Ltd. 5th edition, 2000.
2. Vogels Text book of Quantitative chemical analysis, J.Mendham, R.C Denny, J.D. Barnes, M J.K.Thomas, pearson education, 6th edition, 2002.

REFERENCES

1. Principles of Instrumental Analysis by Skoog, Holler & Nieman Thomson Publishers (2010)
2. Analytical Chemistry – Y.Anjaneylu, K. Chandrasekhar, V. Manickam- Pharma book syndicate, 2007.
3. Instrumental methods of analysis, Willand merrit and dean, caps publications & Distribution, 1999.
4. Instrumentation methods of analysis, Chatwal & Anand, Himalaya Publications, 2003.
5. Principles of Analytical Chemistry by Vacarcel, Springer Publications, 2005

Outcome: The student will able to apply different analytical techniques in the industry