Genetic Engineering Mid - II, November - 2014

1.PCR as a tool is used in generating
  • Site directed mutagenesis
  • Site specific recombination
  • Site specific translocation
  • Transposition.
Answer: A
2.Introduction of DNA into living cells by exposing to an electric pulse is called
  • Electro fusion
  • Electrofision
  • Transfection
  • Electroporation
Answer: D
3.Restriction endonucleases are also known as
  • Molecular scissors
  • Molecular stichers
  • DNA synthesis
  • Polymerases
Answer: A
4.RNA is copied into a complementary form (cDNA) by using
  • TaqDNA polymerase
  • RNA polymerase II
  • Reverse transcriptase
  • Uracil-N-Glycosylase
Answer: C
5.A reverse transcriptase reaction can be primed by using
  • Target sequence specific primers
  • Random hexamers
  • OligodT primers
  • All of the above
Answer: D
6.The technique used to locate a specific gene on a chromosome is
  • Colony hybridization
  • Insitu hybridization
  • Dot blot technique
  • Western Blotting
Answer: B
7.VNTR is an acronym for
  • variable nucleotide triplet repeat
  • variable nucleoside tandem repeat
  • variable nucleoside triplet repeat
  • variable number of tandem repeats
Answer: D
8.RAPD is a
  • DNA sequencing based method
  • Restriction digestion based method
  • PCR basedmethod
  • All of these
Answer: C
9.The DNA microarrays technology thatindicates which genes are transcribed is called
  • DNA variation screening
  • gene expression profiling
  • microarray comparative genomic hybridization
  • antisense
Answer: B
10.In Gene knockout technology
  • A gene is inserted by non-homologous recombination
  • A mutant gene is replaced by a functional copy
  • A functional gene is replaced by a mutant copy
  • A functional gene is inserted in addition to the mutant copy
Answer: C
11.Virus mediated gene transfer is called ___________________
Answer: Transfection
12.________________bacterium is a natural genetic engineer.
Answer: AgrobacteriunTumefaciens
13.Western blotting is the technique used for the detection of _______________
Answer: Protein
14.Commonly used probe for glycoproteins is ________________________
Answer: Lectins
15._______________ are used to select genes of interest from a genomic library.
Answer: DNA probes
16.Protein coding genes can be identified by______________________
Answer: Transposon tagging
17.______________ areoften used for gene therapy to carry a healthy gene.
Answer: Viruses
18._____________and _____________ markers are used for human insulin cloning.
Answer: Antibiotic and B-gal
19.Gene therapy, a technique that helps in _________________________
Answer: Curing genetic disorders
20.Restriction endonucleases recognize specific sequences on DNA called ______________
Answer: Palindromes