Genetic Engineering Mid - I, September - 2014

1.Catabolite activating protein exerts which type of control on transcription of the lac operon genes.
  • positive
  • negative
  • may be positive or negative
  • none of these
Answer: A
2.The DNA that actually codes for a protein's primary structure in eukaryotes is
  • the entire gene
  • portions of the gene called introns
  • portions of the gene called exons
  • the promoter
Answer: C
3.In the presence of high levels of tryptophan
  • attenuator allows transcription of trp structural genes
  • attenuator propogates transcription
  • attenuator terminates transcription
  • none of the above
Answer: C
4.Which type of plasmid can exist with or without being integrated into the host's chromosome?
  • Medisome
  • Lisosome
  • Lysogen
  • Episome
Answer: D
5.Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid?
  • DNA polymerase
  • Restriction endonuclease
  • DNA ligase
  • DNA helicase
Answer: C
6.Which of the following vector can maintain the largest fragment of foreign DNA?
  • YAC
  • Cosmid
  • Plasmid
  • Phage
Answer: A
7.Problems in obtaining large amounts of proteins encoded by recombinant genes can often be overcome by using
  • BACS
  • expression vectors
  • YACS
  • all of these
Answer: B
8.Which of the following enzyme is used to cut DNA molecule internally
  • restriction enzymes
  • restriction endonuclease
  • restriction exonuclease
  • ribonuclease H
Answer: B
9.Single stranded unpaired extensions formed by restrictionenzyme upon cleavage is called as
  • blunt ends
  • flush ends
  • sticky ends
  • non-sticky ends
Answer: C
10.In DNA sequencing, the primer
  • specifies where the sequence ends
  • specifies where the sequence begins
  • breaks the sequence
  • generates variety of different sized fragments
Answer: B
11.Transcription factors are regulatory proteins that bind to a________________
Answer: Promoter site
12.Enhancers are cis-activating, while TFs are ________________
Answer: trans-activating
13.Lac Z gene is associated in the synthesis of _________________ enzyme.
Answer: beta-galactosidase enzyme
14.Small, circular DNA molecules that can exist independently of chromosomes commonly found in bacteria are called ________________
Answer: Plasmid
15.Transposons are also called as ______________.
Answer: Jumping genes
16.Plasmids can be transferred between bacterial hosts through a process known as _____________
Answer: Bacterial conjugation
17._______________ is a thermostable DNA polymerase.
Answer: Ex:Taq polymerase or vent polymerase or pfu polymerase
18.Phage is used as______________.
Answer: Vector
19.Gel electrophoresis separates DNA fragments according to their ______________.
Answer: Size
20.When "sticky ends" are paired, they can be joined by _______________
Answer: DNA ligase